Micrometastases Detection generated by Organoids | Protocol Preview скачать в хорошем качестве

Micrometastases Detection generated by Organoids | Protocol Preview

Watch the Full Video at https://www.jove.com/v/57374/high-sen.... High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor - a 2 minute Preview of the Experimental Protocol Yu Okazawa, Kosuke Mizukoshi, Yu Koyama, Shoki Okubo, Hiromitsu Komiyama, Yutaka Kojima, Michitoshi Goto, Sonoko Habu, Okio Hino, Kazuhiro Sakamoto, Akira Orimo Juntendo University Faculty of Medicine, Department of Coloproctological Surgery; Juntendo University Faculty of Medicine, Department of Molecular Pathogenesis; Juntendo University Faculty of Medicine, Atopy Research Center; Tokyo Dental College, Department of Oral Pathobiological Science and Surgery; Juntendo University Faculty of Medicine, Department of Gastroenterology; To allow highly sensitive detection of the disseminating human colorectal cancer (CRC) cells colonizing tissues, we herein show a protocol for efficient transduction of green fluorescent protein (GFP) lentiviral particles into PDX-derived CRC organoid cells prior to their injection into recipient mice, with stereo-fluorescence microscopic observation. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments