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Soybean Leaf Sampling for Nutritional Needs

It is very important to select the correct soybean leaves when tissue sampling to determine nutritional needs during the growing season. The most recent, mature trifoliate leaf will provide the truest assessment of the plant’s nutrition. This is usually the leaves from the third or fourth nodes from the top of the plant, depending on the development of the upper most trifoliate. If the upper most leaf is less than, or equal to, the size a quarter then pull the trifoliate leaf from the fourth node for your sample. However, if the upper most leaf is equal to, or greater than, the size of a half dollar, then chose the trifoliate leaf from the third node. For each sample submitted to a laboratory for analysis, pull 20-25 trifoliate leaves in the desired area, placed loosely in a paper bag marked with identifiers such as name, field, location, date, and growth stage of the soybean. Do not allow the leaves to remain moist, as they will mold quickly and ruin the sample. If you are tissue sampling multiple times during the season, you should wait at least 10-14 days between samples. This will allow any past foliar fertilizer(s) applied to be incorporated into the plant and assessed. It will also test a new set of nodal leaves that have developed since the last sampling.