Open Multiplexing Transforming Tissue Research with Cell DIVE скачать в хорошем качестве

Open Multiplexing Transforming Tissue Research with Cell DIVE

Cancer is a complex disease at the level of both cells and tissues, and uncovering novel treatments requires understanding the biology and organization of many different cell types and tissues. The ability to properly characterize these different features is limited by the analysis method; genomics methods are incredibly broad, but lack spatial information, and imaging methods frequently lack the breadth to understand multiple interlinked oncological phenomena. Cell DIVE, a complete open multiplex imaging workflow: Here, we present Cell DIVE, a complete open multiplex imaging workflow that allows for the imaging of over sixty biomarkers in a single tissue section through iterative staining and dye inactivation. This allows for multiple areas of cancer biology to be explored simultaneously. First, using Cell DIVE, we show how cancer cells in hypoxic regions of pancreatic tumors can adapt to extreme conditions, evade cell death, and avoid immune surveillance.  Second, we explore how Cell DIVE’s flexible and scalable workflow can grow to meet the needs of your multiplex imaging study while allowing the researcher flexibility in how the workflow is performed.  Last, we demonstrate how automation can be leveraged to improve the speed of complex imaging studies and minimize touch points. By utilizing more biomarkers in an imaging study, the influence and relationships of additional biological pathways on cancer progression can be more easily studied, potentially paving the way for better targeting of emerging therapies. Key Learnings: Learn how crystal-clear whole tissue images enable you to deepen your understanding of the tissue microenvironment. Understand how open multiplexing can help you adapt to ever-changing study requirements. Watch how open multiplexing lets your research dictate the level of automation required, which antibodies to use, how to build your antibody panel and more. 0:00 - Introduction 1:53 - Cell DIVE: Transfoming tissue research with open multiplexing (speakers: Michael Smith and Katie White) 36:55 - Q&A Session #cellbiology #cancerresearch #biomarkers ▬ Learn more ▬▬▬▬▬▬▬▬▬▬▬▬ Learn more about CellDIVE https://fcld.ly/u405a23 Learn more about THUNDER Imaging Systems https://fcld.ly/o25o3tk Discover our Science Lab Articles on Cancer Research https://www.leica-microsystems.com/sc... ▬ More Videos ▬▬▬▬▬▬▬▬▬▬▬▬ Subscribe @LeicaMicrosystems @LeicaMicrosystemsTutorials ▬ Follow us ▬▬▬▬▬▬▬▬▬▬▬▬ Discover us on LinkedIn:   / leica-microsystems   Discover our LinkedIn Medical Channel:   / leica-microsystems-medical   Find us on Facebook:   / leicamicrosystems   Follow us on Twitter:   / leicamicro   Follow us on Instagram:   / leica.microsystems   ▬ About Leica Microsystems ▬▬▬▬▬▬▬▬▬▬▬▬ Leica Microsystems develops and manufactures microscopes and scientific instruments for the analysis of microstructures and nanostructures. Ever since the company started as a family business in the nineteenth century, its instruments have been widely recognized for their optical precision and innovative technology. It is one of the market leaders in compound and stereo microscopy, digital microscopy, confocal laser scanning microscopy with related imaging systems, electron microscopy sample preparation, and surgical microscopes.