Protein Preparation, Animal Vaccination & Neutralization Detection l Protocol Preview скачать в хорошем качестве

Protein Preparation, Animal Vaccination & Neutralization Detection l Protocol Preview

Watch the Full Video at https://www.jove.com/v/2444/protocol-.... Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection - a 2 minute Preview of the Experimental Protocol Lanying Du, Xiujuan Zhang, Jixiang Liu, Shibo Jiang New York Blood Center, Lindsley F. Kimball Research Institute; This protocol describes a general procedure for studying recombinant receptor-binding domain (RBD)-based subunit vaccines against SARS. It includes methods for transfection and expression of RBD protein in 293T cells, immunization of mice with RBD and detection of neutralization activity of mouse sera using an established SARS pseudovirus neutralization assay. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments