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Watch the Full Video at https://www.jove.com/v/56767/establis.... Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma - a 2 minute Preview of the Experimental Protocol Alessandro De Vita, Laura Mercatali, Giacomo Miserocchi, Chiara Liverani, Chiara Spadazzi, Federica Recine, Alberto Bongiovanni, Federica Pieri, Davide Cavaliere, Valentina Fausti, Dino Amadori, Toni Ibrahim Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Osteoncology and Rare Tumors Center; Morgagni-Pierantoni Hospital, Pathology Unit; Morgagni-Pierantoni Hospital, Unit of Surgery and Advanced Oncologic Therapies; The following protocol focuses on the establishment of a primary culture of patient-derived soft tissue sarcoma (STS). This model could help us to better understand the molecular background and pharmacological profile of these rare malignancies and could represent a starting point for further research aimed at improving STS management. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments