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• This monthly support webinar helps all types of researchers utilize the cancer genomics data and resources available at NCI’s Genomic Data Commons (GDC). GDC experts will demonstrate how to find and access genomic data, how to use web-based tools, give in-depth explanations of bioinformatics pipelines, and more. • The GDC processes single-cell RNA-Seq (scRNA-Seq) data using the Cell Ranger pipeline to calculate gene expression, followed by Seurat for secondary expression analysis, and makes the aligned reads file (BAM), as well as raw and filtered counts (MEX) accessible to the research community. Researchers can analyze scRNA-Seq data using the newly developed GDC scRNA-Seq Tool, which streamlines the exploration of single cell RNA sequencing data through cluster plots and gene expression overlays. Expression data can also be accessed programmatically using the new GDC scRNA-Seq gene expression API endpoint. This webinar will provide an in-depth look at GDC scRNA-Seq workflows, demonstrate how the scRNA-Seq Tool can enhance single-cell research efforts, and review the GDC scRNA-Seq gene expression API.