MBIO 3010 0205 скачать в хорошем качестве

MBIO 3010 0205

Table of Contents: 00:01 - Microbiology: An Introduction 00:37 - Topics 00:40 - Metric system Standard unit is meter Units related by 10 Microbes are measured in μm and nm 05:23 - Figure 3.2 Microscopes and Magnification. 09:28 - Microscopy: The Instruments 10:09 - Light microscopes 10:57 - Compound microscope Total magnification = objective lens × ocular lens 16:03 - The path of light. 16:22 - Resolution - ability of the lenses to distinguish two points Resolving power of 0.4 nm  distinguish between two points ≥ 0.4 nm Improve resolution by: Light source: Shorter wavelengths, greater resolution Immersion oil 18:59 - Refractive index light-bending ability of a medium Light bends in air May refract and miss the objective lens Immersion oil Keeps light from refracting Offers better resolution 20:19 - Light Microscopy 21:09 - Brightfield Illumination 22:08 - Darkfield Illumination 23:30 - Phase-Contrast Microscopy 24:32 - Fluorescence Microscopy 25:14 - Confocal Microscopy 26:43 - Electron Microscopy 28:19 - Transmission Electron Microscopy (TEM) 29:39 - Transmission Electron Microscopy (TEM) 31:32 - Scanning Electron Microscopy (SEM) 32:21 - Resolution is 10 nm; magnifies 1,000 to 500,000x 35:40 - Scanning Tunneling Microscopy 36:40 - Atomic Force Microscopy 38:06 - Why do we stain bacteria? Live or unstained microbes have little contrast with surrounding medium Increased contrast allows viewing of cell morphology and other structures Staining: Coloring the microbe with a dye to increase contrast Emphasizes certain structures 39:00 - Preparing Smears for Staining 40:23 - Staining 43:29 - Simple Stains 44:52 - Differential Stains 45:34 - Gram Stain 48:47 - Slide 1 51:24 - Gram stain - Result 53:16 - Acid-Fast Stain 55:44 - Acid-fast bacteria 56:32 - Special Stains 56:41 - Capsule stain 58:00 - Endospore stain 01:01:06 - Flagella stain