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Watch the Full Video at https://www.jove.com/v/2807/a-rapid-a.... A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices - a 2 minute Preview of the Experimental Protocol Jennifer Selever, Jian-Qiang Kong, Benjamin R. Arenkiel Baylor College of Medicine (BCM), Department of Molecular & Human Genetics; Precisionary Instruments Inc.,; Baylor College of Medicine (BCM), Departments of Molecular & Human Genetics and Neuroscience; Texas Children's Hospital, Jan and Dan Duncan Neurological Research Institute; Here we describe a rapid and simple method to image fluorescently labeled cells in semi-thick brain slices. By fixing, slicing, and optically clearing brain tissue we describe how standard epifluorescent or confocal imaging can be used to visualize individual cells and neuronal networks within intact nervous tissue. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments