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High Content Screening (HCS)

High-Content Screening (HCS) refers to a group of experimental approaches that consists in the use of multi-well imaging plates (96 to 384-well plates) to carry cell-based functional assays, allowing multiple treatment conditions to be evaluated simultaneously. The HCS assays are based on the use of fluorescent reagents and automated immunofluorescence microscopy. The automated acquisition of digital images, coupled to their automated processing and analysis, allows diverse molecular and morphological events to be quantified at a single-cell-level. The high-content of these multiparametric readouts, allow diverse complex biological processes to be interrogated simultaneously, following perturbations with different chemical or biological compounds (e.g. unknown or known drug inhibitors or agonists, cytokines, growth factors, etc) or genetic tools (siRNA, esiRNAs, shRNAs, microRNA mimics, microRNA inhibitors, CRISPR sgRNAs, etc). The LLSFBio provides support for all phases involved in a HCS project, including: 1) project evaluation and technical consultation, 2) HCS assay development and validation, 3) lab automation and screening execution, 4) automated image acquisition, 5) quantitative image analysis and 6) Data analysis.