Step-specific Sorting of Spermatids by Flow Cytometry | Protocol Preview скачать в хорошем качестве

Step-specific Sorting of Spermatids by Flow Cytometry | Protocol Preview

Watch the Full Video at https://www.jove.com/v/53379/step-spe.... Step-specific Sorting of Mouse Spermatids by Flow Cytometry - a 2 minute Preview of the Experimental Protocol Olivier Simard, Frédéric Leduc, Geneviève Acteau, Mélina Arguin, Marie-Chantal Grégoire, Marc-André Brazeau, Isabelle Marois, Martin V. Richter, Guylain Boissonneault Université de Sherbrooke, Department of Biochemistry; Université de Sherbrooke, Department of Medicine; We describe a sorting strategy for mouse spermatids using flow cytometry. Spermatids are sorted into four highly pure populations, including round (spermiogenesis steps 1-9), early elongating (spermiogenesis steps 10-12), late elongating (spermiogenesis steps 13-14) and elongated spermatids (spermiogenesis steps 15-16). DNA staining, size and granulosity are used as selection parameters. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments