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In this video, I explain the most important statistical tools used in agricultural and biological research. You will learn how to perform and interpret one-sample and two-sample t-tests, and clearly understand the difference between one-tailed and two-tailed tests. We also demonstrate pairwise comparison methods to identify significant differences between treatments. In addition, this tutorial covers the concept of p-value and probability distribution, helping you understand how statistical decisions are made. Finally, we introduce linear regression, showing how to analyze relationships between variables and interpret regression equations and R² values in R. This video is especially useful for students, researchers, and beginners who want to analyze experimental data using R in agriculture, biology, and related fields. t-test (one sample & two sample) One-tailed vs Two-tailed test Pairwise comparison p-value and distribution Linear regression