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There are numerous methods to treat the lipoaspirate, to obtain only the fat cells that matter for the re-injection (the adipose tissue). This is known as Fat Purification or Fat Processing. Each specialist might have their preferred fat processing method. A number of specialists advocate for washing the fat with a saline solution, while others prefer centrifugation. Some even combine methods. Here Dr. Kayser shows a combination of two methods. Decantation, first he lets the lipoaspirate sediment into 4 layers : (1) desired fat cells, but also other liquids such as (2) blood, (3) tumescent fluid and (4) ruptured fat cells that form an oily layer. Centrifugation, then he uses a manual centrifugation machine - he can control manually the centrifugation speed. This provides an additional filter and in the end, more blood and tumescent solution are separated at the bottom, leaving a nice fat layer at the top of the syringes. There is no agreement on which is the best way to purify the fat to assure its maximum viability (a). Also the latest medical research in fat grafting has shown that there is no single best cell-processing technique (b). Research sources: a. Alexandra Condé-Green, Natale Ferreira Gontijo de Amorim, Ivo Pitanguy, Influence of decantation, washing and centrifugation on adipocyte and mesenchymal stem cell content of aspirated adipose tissue: A comparative study, Journal of Plastic, Reconstructive & Aesthetic Surgery, Volume 63, Issue 8, 2010, Pages 1375-1381 b. Wolters Kluwer Health. "Best way to prepare fat cells for grafting? Jury's still out." ScienceDaily. ScienceDaily, 28 September 2015. www.sciencedaily.com/releases/2015/09/150928152448.htm