Maximizing his-tag protein binding with HisTrap™ excel columns скачать в хорошем качестве

Maximizing his-tag protein binding with HisTrap™ excel columns

Protein purification can be something of an art, where even the smallest change to a protocol can have a big impact on the results. Achieve optimal results in his-tag protein purification with HisTrap™ excel columns, designed to maximize protein binding while minimizing Ni-leakage. In this video, discover how small adjustments in your protocol can significantly improve purification outcomes and ensure the highest quality for your target proteins. What you’ll learn in this video: • How HisTrap columns enhance binding efficiency for his-tag proteins. • The advantages of using HisTrap excel over conventional IMAC media. • Practical tips for optimizing his-tag protein purification workflows. HisTrap excel columns are prepacked with a high-performance ligand to ensure consistent and reliable purification of his-tagged proteins. Whether you're performing small-scale research or scaling up for production, these columns simplify the purification process while delivering reproducible results. Learn more about HisTrap excel columns: https://www.cytivalifesciences.com/en... Explore protein purification techniques: https://www.cytivalifesciences.com/en... Elevate your his-tag purification workflows with HisTrap excel columns and take your protein research to the next level. Chapters: 00:00:00 Introduction 00:00:17 Utilization of histidine tag proteins 00:00:20 Troubleshooting 00:00:34 Issues in finding files 00:00:38 Protein discussion 00:00:45 Lessons from the past year 00:00:47 The role of EDTA in sample processing 00:00:51 Challenges with His-tag column binding 00:01:00 Media and Trap XL: minimizing nickel leakage 00:01:05 Maximizing pure protein yield 00:01:09 Presence of stripping agents #chromatography #biopharma #lifesciences