Quick guide to interpreting and drawing amino acid titration curves скачать в хорошем качестве

Quick guide to interpreting and drawing amino acid titration curves

I know you all have a lot on your plates, so I’m going to work on being more concise, starting with this “quick” (for me at least) guide to interpreting and drawing amino acid titration curves blog: https://bit.ly/aminoacidtitrationcurves Strategy for drawing amino acid titration curves:  1. Identify # of ionizable groups  at least 2 (N- & C-termini), possibly 3 (R group)  this #= # of OH⁻ equivalents (eq.) required to fully deprotonate it  2. Draw your x-axis, extending that many equivalents-worth  label each 0.5 increment  3. Sketch the state of each ionizable group at each increment & label its charge  remember groups will deprotonate in order of increasing pKa (most acidic first)  4. Plot points intersecting each at 1/2-way between equivalents OH⁻ (0.5, 1.5, 2.5 - if applicable)   the corresponding pH = pKa, so [HA] = [A⁻]   5. Draw a flattish "buffering zone" around this point  6. Draw points at each 1 OH⁻ (0, 1, 2, 3 (if applicable)) halfway between the the pKa’s on either side  the steepest part of the inflection will be here  one of these pH’s will be the pI  7. Connect the dots and lines, drawing the steepest part of the inflection curves through these equivalence points 8. Label the pI (pH at 0 net charge point)  will be the average of the pKa’s on either side of it  average of the 2 most similar pIs     If you want a longer video, with more worked examples:    • Drawing titration curves for amino acids -...     At 19:40, I show an example of how to draw an amino acid titration curve for an amino acid with a non-ionizable R group (in this case glycine).    • Drawing titration curves for amino acids -...     At 30:14, I show an example of how to draw an amino acid titration curve for an amino acid with a basic R group (in this case lysine)    • Drawing titration curves for amino acids -...     At 40:22, I show an example of how to draw an amino acid titration curve for an amino acid with an acidic R group (in this case glutamate)    • Drawing titration curves for amino acids -...      At 49:30, I give a summary and note that titration curves are nice for some things, especially helping you get a sense as to what’s going on at different pH’s, but if you want to actually calculate what the pH would be or what proportion would be protonated at a given pH, for an in-between equivalents point, the Henderson-Hasselbach equation is the place to go!  http://bit.ly/phbuffers YouTube:    • Henderson-Hasselbalch equation = where it ...      P.S. Huge thanks to Dr. Jeff Sigman for helping teach me his method!   Much more on amino acids: http://bit.ly/allaminoacids ; YouTube:    • Amino acids - all about 'em!       More on acids, bases, & pKa calculations:   http://bit.ly/phbuffers YouTube:    • Henderson-Hasselbalch equation = where it ...   &    • Видео        more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 http://bit.ly/2OllAB0 or search blog: http://thebumblingbiochemist.com