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It has a history of taxonomic chaos, jumping from genus to genus for over a century. It's a major veterinary pathogen causing devastating economic losses on farms, and it's an increasingly recognized cause of severe human infections, from abscesses to fatal endocarditis. Meet Trueperella pyogenes. This comprehensive guide is for every clinical microbiologist who has seen those characteristic tiny colonies with massive beta-hemolysis and wondered about the full story. We'll trace its winding taxonomic journey, explore the complete disease spectrum from livestock to humans, and detail the entire laboratory identification workflow. Learn the crucial bench clues—from its pleomorphic Gram stain to its key biochemical reactions like reverse CAMP. We'll also cover the challenges of identification with MALDI-TOF, the importance of molecular confirmation by targeting the plo gene, and how to navigate the complex world of susceptibility reporting in the absence of specific EUCAST breakpoints. This video was made with Gemini's deep research capabilities and NotebookLM. Explore our research and sources for this video in our NotebookLM document: https://notebooklm.google.com/noteboo... Timestamps: 00:00 - Introduction: More Than Beta-Hemolysis 00:38 - Chapter 1: A Pathogen's Identity 01:45 - Chapter 2: From Farm to Clinic 02:44 - Chapter 3: Laboratory Identification 04:34 - Chapter 4: The Reporting Dilemma 06:03 - Chapter 5: Treatment & Prevention Like this video for more deep dives into clinical bacteriology, and please subscribe to the channel! How does your lab handle susceptibility reporting for organisms without specific breakpoints? We'd love to hear your approach in the comments below. #TrueperellaPyogenes #Bacteriology #Microbiology #Zoonosis #InfectiousDisease #VeterinaryMedicine #OneHealth #ClinicalMicrobiology #LabDiagnostics #AntibioticResistance #Endocarditis #Abscess #MedicalEducation #Actinomyces #EUCAST