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Do you have to equilibrate a HILIC column more than a reversed phase column? | Trust Your Science, 5 лет назад


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Do you have to equilibrate a HILIC column more than a reversed phase column? | Trust Your Science,

HILIC (hydrophilic Interaction Liquid Chromatography) can be a challenging separation technique especially for those who are more familiar with reversed phase chromatography. Often with HILIC, there is poor retention time reproducibility from injection to injection as the retention times of the peaks drift. In this episode of #Trust Your Science, Jonathan and Kim take a closer look at the effect equilibration has on HILIC reproducibility, and weather or not you need to equilibrate your HILIC columns longer than reversed phase.

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