Fluorescence Minus Multiple (FMM) Basics скачать в хорошем качестве

Fluorescence Minus Multiple (FMM) Basics

Researchers use Fluorescence Minus Multiple (FMM) controls to assess and minimize signal "spillover" between fluorophores in a cytometry panel. Including FMM controls in experiments helps ensure that the channels on a cytometry panel accurately detect their target markers. This week's YouTube video covers a brief overview of FMM and its use in cytometry. Tune in for an overview of: How signal spillover can produce artifacts (i.e. false signals) in the emission spectrum of fluorophores. How FMM serves as an alternative to Fluorescence Minus One (FMO) controls, saving researchers time and money while yielding similar results. How the removal of fluorophores is distributed across FMM controls, according to published experiment design considerations. 0:00 Introduction 0:29 Who Cares? 0:42 Emission Spectra Overlap in Spectral Cytometry 1:51 Measuring Spillover 3:12 Fluorescence Minus One Controls 3:53 FMM Control Design 5:40 FMM in Action 6:09 FMM Results from Teiko's Spectral Panel #immunotherapy #cytometry #FMM #FMO #fluorophore #clinicaltrial #lifescience #validation #paneldesign