How to Deal with Old Tissue Culture Plants скачать в хорошем качестве

How to Deal with Old Tissue Culture Plants

Old tissue culture plants don’t have to be thrown away. In this video, I show how to rescue overgrown, declining in-vitro cultures by restarting them on fresh media. Using Impatiens repens and Vitis sultana (grape) as examples, I walk through how to open old culture vessels, identify healthy tissue, take clean nodal cuttings, and transfer them into new media to restore vigorous growth. This is a practical reset technique used when cultures have been sitting too long, are nutrient-depleted, elongated, or close to crashing—but still salvageable. This method works for both herbaceous and woody species and is a normal part of long-term tissue culture management, not a failure. If there’s still healthy tissue left, there’s still a way to save the plant. Perfect for anyone working with plant tissue culture, micropropagation labs, or maintaining long-term in-vitro collections.