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Watch the Full Video at https://www.jove.com/v/58428/automati.... Automation of a Positron-emission Tomography (PET) Radiotracer Synthesis Protocol for Clinical Production - a 2 minute Preview of the Experimental Protocol Eric Schopf, Christopher M. Waldmann, Jeffrey Collins, Christopher Drake, Roger Slavik, R. Michael van Dam SOFIE,; David Geffen School of Medicine, University of California, Los Angeles (UCLA), Department of Molecular & Medical Pharmacology; University of California, Los Angeles (UCLA), Ahmanson Translational Imaging Division; University of California, Los Angeles (UCLA), Crump Institute for Molecular Imaging; Positron-emission tomography (PET) imaging sites that are involved in multiple early clinical research trials need robust and versatile radiotracer manufacturing capabilities. Using the radiotracer [18F]Clofarabine as an example, we illustrate how to automate the synthesis of a radiotracer using a flexible, cassette-based radiosynthesizer and validate the synthesis for clinical use. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments