How to calculate Protein concentration using standard curve of BCA assay ? Easy steps скачать в хорошем качестве

How to calculate Protein concentration using standard curve of BCA assay ? Easy steps

In this video, you will how to construct a Standard curve of BCA protein assay, and how to calculate protein concentration using a standard curve. The Micro Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent-solubilized protein solutions and is a modification of the Bicinchoninic Acid (BCA) Protein Assay for dilute protein samples (0.5-20µg/ml). The Micro Bicinchoninic Acid (BCA) Protein Assay primarily relies on two reactions. Firstly, the peptide bonds in the protein sample reduce Cu2+ ions, in a temperature-dependent reaction, from the copper solution to Cu+ . The amount of Cu2+ reduced is proportional to the amount of protein present in the solution. Next, two molecules of bicinchoninic acid (BCA) chelate with each Cu+ ion, forming a purple-colored product that strongly absorbs light at a wavelength of 562 nm. The Micro BCA Assay uses concentrated solutions and extended incubation times for the detection of dilute protein samples. The amount of protein present in a solution can be quantified by measuring the absorption spectra and comparing protein solutions with known concentrations. For the BCA Assay procedure please watch this video:    • How to perform Protein quantification by B...   Please subscribe to my channel for more useful videos about biological methodology and science-related videos. Music Credit: https://www.youtube.com/watch?v=eELeR... BCA Assay Standard Curve construction Calculation of protein concentration using a standard curve How to construct standard curve in Skanlt Software How to construct a Standard curve in Microsoft Excel #BCA_Assay_Standard_Curve #BioCapsule #Protein_Quantification License