3D Fluorescence Microscopy: Imaging Of Viral Transcription Factories скачать в хорошем качестве

3D Fluorescence Microscopy: Imaging Of Viral Transcription Factories

Watch the Full Video at https://www.jove.com/v/56832/function.... Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy - a 2 minute Preview of the Experimental Protocol Christopher P. Chen, Frank Chuang, Yoshihiro Izumiya University of California, Davis, Department of Dermatology, University of California Davis School of Medicine; University of California, Davis, Center for Biophotonics, Department of Biochemistry and Molecular Medicine; Viral transcriptional factories are discrete structures that are enriched with cellular RNA polymerase II to increase viral gene transcription during reactivation. Here, a method to locate sites of actively transcribing viral chromatin in 3D nuclear space by a combination of immunofluorescence staining and in situ RNA hybridization is described. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments