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CHEMetrics 3-minute procedure uses the standard methyl orange chemistry and features a unique extraction technique. The extraction eliminates several steps required in other procedures and provides increased sensitivity. The filming amine compound reacts with methyl orange to form a yellow-colored complex that is extracted into an immiscible organic solvent. Results are expressed in ppm (mg/L) octadecylamine. Directions for conducting the test seen here: Rinse the reaction tube with the sample to be tested. (RINSE) Fill it to the 10 mL mark with the sample. (FILL) While holding the double-tipped ampoule in a vertical position, snap the upper tip using the tip breaking tool. (SNAP FIRST TIP) Invert the ampoule and position the open end over the reaction tube. (INVERT) Snap the upper tip. (SNAP SECOND TIP) Allow the contents to drain into the reaction tube. (DRAIN) Cap the reaction tube and shake it vigorously for 1 minute. (SHAKE – ONE MINUTE) Allow the tube to stand undisturbed for 1 minute. (STAND – ONE MINUTE) Make sure the flexible tubing is firmly attached to the CHEMet ampoule tip and insert the CHEMet assembly (tubing first) into the reaction tube making sure that the end of the flexible tubing is at the bottom of the tube. (INSERT CHEMET) Break the tip of the CHEMet ampoule by gently pressing it against the side of the reaction tube. The ampoule should draw in fluid only from the organic phase (bottom layer). (SNAP CHEMET TIP) When filling is complete, remove the CHEMet assembly from the reaction tube. (REMOVE CHEMET) Remove the flexible tubing from the CHEMet ampoule. Dry the ampoule, then place a cap firmly onto the ampoule tip. (REMOVE & DRY) Invert the ampoule several times, allowing the bubble to travel from end to end. (INVERT) Place the ampoule, flat end first, into the comparator. Hold the comparator up toward a source of light and view from the bottom. (COMPARE) Rotate the comparator until the best color match is found. (OBTAIN RESULT)