FACS used for Isolation of Human Lymphatic Endothelial Cells | Protocol Preview скачать в хорошем качестве

FACS used for Isolation of Human Lymphatic Endothelial Cells | Protocol Preview

Watch the Full Video at https://www.jove.com/v/52691/isolatio.... Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting - a 2 minute Preview of the Experimental Protocol Zerina Lokmic, Elizabeth S. Ng, Matthew Burton, Edouard G. Stanley, Anthony J. Penington, Andrew G. Elefanty The Royal Children’s Hospital, Murdoch Childrens Research Institute; The University of Melbourne, Department of Paediatrics, Faculty of Medicine, Dentistry and Health Sciences; Monash University, Clayton, Department of Anatomy and Developmental Biology, Faculty of Medicine, Nursing and Health Sciences; The goal of this protocol is to isolate lymphatic endothelial cells lining human lymphatic malformation cyst-like vessels and foreskins using fluorescence-activated cell sorting (FACS). Subsequent cell culturing and expansion of these cells permits a new level of experimental sophistication for genetic, proteomic, functional and cell differentiation studies. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments