Mikhail Dozmorov, Workshop 500: Differentially interacting chromatin regions from multiple Hi-C data скачать в хорошем качестве

Mikhail Dozmorov, Workshop 500: Differentially interacting chromatin regions from multiple Hi-C data

500: Detection of differentially interacting chromatin regions from multiple Hi-C datasets Mikhail Dozmorov (Virginia Commonwealth University) 10:00 AM - 10:55 AM EDT on Thursday, 30 July WORKSHOP This is an introductory workshop to comparative Hi-C data analysis. The format of the class consists of an introductory lecture followed by hands-on practical examples. We will outline steps necessary for raw FASTQ data processing in order to obtain Hi-C contact matrices. The principles of joint normalization will be discussed, along with statistical tests applied to detect statistically significant differences in chromatin interaction frequencies between two or more Hi-C datasets. Participants will learn how to change between various Hi-C data formats. As part of the lab session, participants will perform Hi-C data normalization and generate a list of regions with significantly different interaction frequencies. We will conclude with examples on how to visualize and interpret the results. Moderator: Aedin Culhane