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Watch the Full Video at https://www.jove.com/v/62868/a-three-.... A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma - a 2 minute Preview of the Experimental Protocol Marco Y. W. Zaki, Shishir Shetty, Alex L. Wilkinson, Daniel A. Patten, Fiona Oakley, Helen Reeves Minia University, Department of Biochemistry, Faculty of Pharmacy; University of Birmingham, National Institute for Health Research Birmingham Liver Biomedical Research Unit and Centre for Liver and Gastrointestinal Research, Institute of Immunology and Immunotherapy; Newcastle University, Newcastle Fibrosis Research Group, Biosciences Institute, Faculty of Medical Sciences; Newcastle University, Newcastle University Translational and Clinical Research Institute, Faculty of Medical Sciences; Newcastle-upon-Tyne Hospitals NHS Foundation Trust, The Liver Unit, Department of Medicine, Freeman Hospital; Comprehensive in vitro models that faithfully recapitulate the relevant human disease are lacking. The current study presents three-dimensional (3D) tumor spheroid creation and culture, a reliable in vitro tool to study the tumor-stromal interaction in human hepatocellular carcinoma. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments