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Sister chromatids and Homologous Chromosomes During interphase of the cell cycle, the DNA is replicated. As a result, two sister chromatids are created. The sister chromatids are identical strands of DNA. During mitosis, the sister chromatids are separated and during meiosis homologous chromosomes pair up. Additional facts about Sister Chromatids and Homologous Chromosomes Sister chromatids are the same size Have the same genes Genes are at the same location or loci on the chromosome Have the same alleles and are held together by a centromere Homologous chromosomes Same size Carry same genes in same loci but can have different alleles because one half is from the female and one half is from the same male ( maternal and paternal). Homologous Chromosomes are not held together by a centromere You may also enjoy... • Diploid Cell vs Haploid Cell