Filtering biochemical solutions - practical advice скачать в хорошем качестве

Filtering biochemical solutions - practical advice

We filter solutions a lot in biochemistry - for sterilization (0.2μm (micron) ones for that) or just de-crudding stuff (often 0.45μm). To get the liquid to go through the membrane we can use syringes, vacuum, or centrifugation (but don't confuse the spin filters with the spin concentrators (ultrafiltration devices). We choose filters based on what we're trying to keep out, what we want to go through safely, and what our solvent is. When we're dealing with protein solutions, we often use a treated PVDF or PES membrane, which have low protein binding. For small volumes we can use spin filters or syringe filters, and for larger volumes, bottle or tube-top vacuum filters. Here are some good guides to choosing How to Select a Syringe Filter and How to Use it? (2020 Guide), Aireka cells, https://airekacells.com/blog/syringe-...   Syringe Filters for Aqueous Solutions, Tisch Scientific https://scientificfilters.com/syringe... Corning filtration guide: https://www.corning.com/catalog/cls/d... here's more on the concentrators - where you don't want the protein to go through: https://bit.ly/spinconcentrators ; new video:    • Centrifugal ultrafiltration (spin concentr...     For more practical protein-purification posts (and background/theory), check out the page on my blog where I’ve collected some of my protein purification posts. http://bit.ly/proteinpurificationtech   and here's the source for the particle size figure: https://commons.wikimedia.org/wiki/Fi... Jisaac9, Mieszko the first, CC BY-SA 3.0 https://creativecommons.org/licenses/... , via Wikimedia Commons more (hopefully) helpful random practical lab tips & tricks: https://bit.ly/lab_tricks_page      more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 http://bit.ly/2OllAB0 or search blog: http://thebumblingbiochemist.com