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If you have questions or comments please go to the new channel Ciencia y Science and ask your questions there so that I can answer them. / @cienciayscience The cDNA synthesis tutorial video is the follow up step after isolating RNA. We are able to synthesize cDNA from RNA through a process called reverse transcription (RT). RT is a process that does not occur in mammalian cells and it is not part of the Central Dogma in Biology. In order to perform RT we make use of a retrovirus enzyme called reverse transcriptase, which is able to synthesize cDNA from RNA. [Time stamps] 0:00 - introduction 3:43 - components 5:17 - measuring RNA concentration 6:26 - loading samples onto Take3 Trio plate 8:27 - BioTek Cytation5 software 12:00 - Calculations to run cDNA synthesis 16:33 - Mixing all components for RT 28:22 - Running RT using thermocycler Links to other tutorial videos associated with this video 1) Isolating RNA from primary cells • Isolating RNA from primary cells 2) Running qPCR of cDNA • Running qPCR of cDNA