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The ESP (Environmental Sample Processor) is a "lab in a can" designed for autonomous deployment. It collects water samples, concentrates particulate material, runs molecular diagnostics that are customized to the needs of the users, and sends data in near real-time. The ESPniagara, operated in the Great Lakes, is the first ever ESP used in a freshwater system. It provides managers an earlier warning of algal blooms and their toxicity, which will enable communities to take action to protect public health sooner. Shown here at the Great Lakes Environmental Research Laboratory (GLERL) are the final steps taken by scientists to get the ESPniagara ready for deployment in Lake Erie (which occurred on July 11, 2017). Support for this work is provided by scientists and technicians from GLERL, the Cooperative Institute for Great Lake Research (CIGLR), and the Monterey Bay Aquarium Research Institute (MBARI). When the prep work has been completed, the ESP is lowered into a can using an overhead crane, guided by Emily Davenport (CIGLR), Kyle Beadle (GLERL), and Roman Marin (MBARI). The can serves as a pressure vessel to protect the ESP when submerged underwater. To seal the can, o-rings are installed by Roman and Emily to ensure that the seal is water tight. To check for a complete seal, helium is then pumped into the can as a way to detect leaks.