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Here we write a simple method to measure plates of a column in Unicorn for AKTA Pure Chromatography Systems. Plates are an excellent way to determine how good your column is. Links to videos I talked about: Introduction to chromatography plates: • How good is your chromatography column? Understanding blocks in Unicorn: • Understanding blocks in Unicorn softw... Reading Unicorn instructions: • Reading Unicorn Language With AKTA Ch... Making a custom column: • Making a Custom Column in Unicorn Chr... Synchronizing blocks: • Synchronizing blocks in Unicorn Chrom... For further reading generally on how to use the AKTA Pure see: https://cdn.cytivalifesciences.com/ap... For more reading on creating methods: https://cdn.cytivalifesciences.com/ap... Please leave a comment below and good luck with your purifications! I don't work for Cytiva & I do not represent them in any way. The view's expressed here are my own. Reach me at my Linkedin page / chad-blamey-ph-d-649a336 ================================ Here's the method for you to use if you want it. Main method: 0.00 Base: CV, Vc=318.557 {ml}, (HiLoad 26/60 Superdex 200 pg)#Column type 0.00 Phase: Method Settings 0.00 Base: SameAsMain 0.00 Alarm pressure: 0.50 {MPa}, 0.00 {MPa} 0.00 Noise reduction UV: 5.0 {sec} 0.00 Injection valve: Load 0.00 Outlet valve: Waste 0.00 Alarm air sensor: Enabled 0.00 Pump wash: (On)#Wash Inlet A, Off, Off, Off 0.00 Flow: (2.60)#Flow rate {ml/min}, On 0.00 End_Block 0.00 Phase: Column Equilibration 0.00 Base: SameAsMain (1.00)#Column Equilibration End_Block 0.00 Phase: Salt Injection 0.00 Base: SameAsMain 0.00 Injection valve: Inject (0.02)#Injection Volumn % Column Injection valve: Load 0.02 End_Block 0.00 Phase: Run 0.00 Base: SameAsMain (1.50)#Length of Run End_Block Music form Aesthetic Chill Lofi Beats: No Copyright Free Relaxing Vlog Music for Easygoing Study • 🌿 Aesthetic Chill Lofi Beats: No Copy... (Last 17 minutes) Everything was written by Massbeats https://freetouse.com/music/massobeats Your music is great, thanks for letting me use it!