BBI International Webinar Series - Professor Claudia Vickers (CSIRO) скачать в хорошем качестве

BBI International Webinar Series - Professor Claudia Vickers (CSIRO)

The Bristol BioDesign Institute's International Webinar Series has been designed as a platform to hear from the best international speakers who are aligned to our core areas of interest. These include: biomolecular design and assembly in the cell, development and delivery of bioactive molecules, minimal biology towards cell-like systems, advanced computing and digital biology. (http://www.bristol.ac.uk/biodesign-in...) In this webinar, Professor Claudia Vickers (CSIRO) gives a presentation on: 'Synthetic biology tools for metabolic engineering in microbes: Isopremoids as a model system'. The event was co-hosted by University of Bristol academics Dr Thomas Gorochowski and Professor Dek Woolfson. 00:00 - Introduction by Dr Thomas Gorochowski 03:07 - Presentation by Prof Claudia Vickers 38:04 - Q&A Abstract We use synthetic biology to (a) understand metabolic regulation of the isoprenoid group of natural products, and (b) engineer production of industrially useful biochemicals. The overall aim of the program is to develop a sufficiently complete understanding of biology such that predictable, reproducible engineering can be achieved. Isoprenoids (terpenes/terpenoids) represent an ideal model system to investigate this challenge. Isoprenoids are an extremely large and diverse group of natural compounds with myriad biological functions, including roles in photosynthesis, respiration, signalling, membrane biosynthesis, and as pigments, hormones, virulence factors, vitamins, etc. They also have many industrial uses, ranging from specialized applications (e.g. anti-cancer and anti-malarial pharmaceuticals, nutraceuticals) through to bulk chemicals (e.g., food colours, fragrances, rubbers, agricultural chemicals, and fuel replacements). However, extracting these compounds from natural sources or chemically synthesizing them is often unfeasible, making them ideal targets for metabolic engineering. We have developed new tools and techniques that help us understand metabolic regulation of pathway flux and achieve controlled cellular behaviour. These include tools for inserting large amounts of DNA onto chromosomes, multi-gene yeast expression vectors, modules to control cell density-dependent gene expression, plant transformation vectors and reporter systems, novel approaches to engineer pathway flux, and subcellular nano-compartments for biocatalysis. This presentation will detail the available synthetic biology tools with examples of their application in isoprenoid production. I will also introduce our high throughput bioengineering facility – the CSIRO BioFoundry. You can find information about upcoming speakers in the Bristol BioDesign Institute's International Webinar Series at: http://www.bristol.ac.uk/biodesign-in....