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Tangential flow filtration (TFF), also known as cross flow filtration, is a rapid and efficient method for the separation and purification of biomolecules. The process fluid passes tangentially across the surface of a filter membrane, and as a pressure differential is applied to the system, constituents in the sample that are small enough to travel through the pore structure of the membrane will pass into the filtrate. Larger constituents will be retained and recirculate around the flow path of the system. The method can be applied to a wide range of biological fields such as immunology, protein chemistry, molecular biology, biochemistry, and biotechnology. TFF can be used to concentrate and desalt sample solutions ranging in volumes from 10 mL to thousands of liters. It can be used to fractionate large from small biomolecules, harvest cell suspensions, and clarify fermentation broths and cell lysates. For laboratory TFF applications the Pall Minimate EVO TFF System offers an easy to assemble and simple to use solution for processing samples up to 1 L. The plug-and-play benchtop TFF system is designed to work with Minimate TFF capsules which are available in a wide range of MWCOs. The reservoir design of the Minimate EVO TFF System allows for either continuous or discontinuous diafiltration to be performed in the same system without sample transfer, allowing for a simplified buffer exchange, reducing the potential for sample loss and handling challenges. In this video you will learn more about the Minimate EVO TFF System, the assembly of the system and how to perform a continuous diafiltration. Learn more: https://www.pall.com/en/laboratory/ta... https://shop.pall.com/us/en/laborator... Download the TFF eBook: https://go.pall.com/cellular-tffebook... Products are designed for research use only. The Auto-translate feature should not be considered exact and in some cases may include incorrect or offensive language. Pall Corporation does not warrant the accuracy, of any information translated by the Auto Translate feature and will not accept liability for loss incurred which results from reliance on such inaccurate translation. Music in this video Song: I Promise Artist: Ian Post Licenced by Artlist (on behalf of Polymorphic Marketing Ltd)