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Watch the Full Video at https://www.jove.com/v/54148/developm.... Development of a Multicellular Three-dimensional Organotypic Model of the Human Intestinal Mucosa Grown Under Microgravity - a 2 minute Preview of the Experimental Protocol Rosangela Salerno-Goncalves, Alessio Fasano, Marcelo B. Sztein University of Maryland School of Medicine, Center for Vaccine Development, Department of Pediatrics; Massachusetts General Hospital for Children, Mucosal Immunology and Biology Research Center; Cells growing in a three-dimensional (3-D) environment represent a marked improvement over cell cultivation in 2-D environments (e.g., flasks or dishes). Here we describe the development of a multicellular 3-D organotypic model of the human intestinal mucosa cultured under microgravity provided by rotating-wall-vessel (RWV) bioreactors. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments