TCGA Samples + Laser Capture Microdissection (LCM) | скачать в хорошем качестве

TCGA Samples + Laser Capture Microdissection (LCM) |

What is #LabChat?    • Welcome to #LabChat - Real Stories from th...   Learn more about the Center for Applied Proteomics and Molecular Medicine: http://capmm.gmu.edu Read Chip's paper: http://cancerres.aacrjournals.org/con... Learn more about the Arcturus LCM system: http://www.lifetechnologies.com/us/en... Dr. Chip Petricoin’s team at the Center for Applied Proteomics and Molecular Medicine at George Mason University had a hunch that the highly pure tumor samples from The Cancer Genome Atlas project (TCGA) were affected by the unknown and unknowable amounts of stroma and other cells. To get to the bottom of this, Chip’s team decided to employ laser capture microdissection (LCM) for their sample prep to see if it made a difference in final output. It turns out that even with TCGA tumor samples that were high in purity (as high as 90%), the phosphoprotein and protein measurements from the microdissected samples showed better statistical concordance with expected genomic arrangements. These results were often entirely lost in undissected sample material. Not only does Chip’s team feel LCM is essential for sample prep, but they’re big fans of the Arcturus laser capture microdissection system and its infrared laser beam. Other platforms solely use a UV laser, which can leave your samples extra crispy, destroying vital RNA and proteins.