Flow cytometry-assisted antimicrobial susceptibility testing using the Attune NxT Flow Cytometer скачать в хорошем качестве

Flow cytometry-assisted antimicrobial susceptibility testing using the Attune NxT Flow Cytometer

https://www.thermofisher.com/us/en/ho... The FAST Method takes 80% less time than current international standard. See how Dr Tim Inglis and his team are advancing antimicrobial susceptibility testing using the Invitrogen Attune NxT Flow Cytometer. * * * * Dr. Tim Inglis, School of Medicine, University of Western Australia, PathWest Laboratory Medicine, discusses the FAST method of antibiotic resistance detection on antimicrobial susceptibility testing using the Invitrogen Attune NxT Flow Cytometer. The Attune NxT analyzes bacteria at a single cell level, and in combination with a range of dyes has expanded the potential repertoire of culture-independent bacteriology well beyond the boundaries that are set by molecular biology and real-time PCR. The FAST method measures the change that is precipitated by exposure to different concentrations of antibiotics, very similar to but much faster than the conventional culture-based methods of minimum inhibitory concentration (MIC) determination. The time differential between FAST MIC and the international reference standard broth microdilution MIC, is approximately 18-24 hours (up to an 80% reduction in time). What would normally take for the international standard 24 hours takes the FAST method using the Attune NxT Flow Cytometer just less than three hours with manual fluid handling. A qualitative result can be obtained, in as little as less than 30 minutes. The principal advantage of the Attune NxT Flow Cytometer is accuracy in the bacterial particle size range, with the ability to do accurate cellular analysis across a range of different acquisition wave lengths. The FAST method was first described in Mulroney and Hall, et al.