From microbial immunity to genome editing скачать в хорошем качестве

From microbial immunity to genome editing

From microbial immunity to genome editing Air date: Wednesday, June 28, 2017, 3:00:00 PM Category: WALS - Wednesday Afternoon Lectures Runtime: 01:06:44 Description: NIH Director's Wednesday Afternoon Lecture Series The microbial CRISPR-Cas adaptive immune systems provide archaea and bacteria with a programmable defense against invading nucleic acids. Several years ago, Dr. Zhang and others reported that the effector module from Streptococcus pyogenes CRISPR-Cas9 (SpCas9) could be harnessed for genome editing. Following this work, Zhang's lab has discovered, characterized, and engineered additional Cas enyzmes for use as molecular biology tools. These include a novel class of CRISPR-Cas systems that use RNA-guided RNases, such as Cas13a (C2c2) and Cas13b. Leveraging the natural properties of Cas13a, the lab developed a single-molecule nucleic acid sensing platform termed SHERLOCK, which can be used for rapid pathogen detection and genotyping. Zhang is continuing to explore microbial diversity to identify novel enzymes and systems that could serve as the basis for the development of additional molecular biology tools and applying these tools to tackle complex biological questions.. For more information go to https://oir.nih.gov/wals Author: Feng Zhang, Ph.D., Core Member, The Broad Institute of MIT; Investigator, McGovern Institute for Brain Research; Associate Professor, Departments of Brain and Cognitive Sciences and Biological Engineering, Massachusetts Institute of Technology Permanent link: https://videocast.nih.gov/launch.asp?...