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Centrifugation Techniques

This video introduces centrifugation. It explains how particles are separated based on size, density, and shape using centrifugal force, and walks through the techniques commonly used in biochemistry and molecular biology labs. In this video you’ll learn: • The core principle of sedimentation under high-speed rotation • Step-by-step workflow for preparing samples, balancing tubes, and running a centrifuge • Distinctions between differential, density-gradient, and ultracentrifugation • How gradients (sucrose, CsCl) improve resolution during separation • How to interpret pellet and supernatant fractions • Applications in cell fractionation, nucleic acid isolation, protein purification, and clinical sample processing The explanation follows a simple, progressive structure—from the principle to the types of centrifugation, to real lab workflows and major research uses. Like, subscribe, and share if this helped you master centrifugation techniques.