Baculovirus Expression Vectors Modificationto Produce Plant Proteins | Protocol Preview скачать в хорошем качестве

Baculovirus Expression Vectors Modificationto Produce Plant Proteins | Protocol Preview

Watch the Full Video at https://www.jove.com/v/58283/modifyin.... Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells - a 2 minute Preview of the Experimental Protocol Sayan Chakraborty, Krittin Trihemasava, Guozhou Xu North Carolina State University, Department of Molecular and Structural Biochemistry, College of Agriculture and Life Sciences; Here, we present the protocols for utilizing the insect cell and baculovirus protein expression system to produce large quantities of plant secreted proteins for protein crystallization. A baculovirus expression vector has been modified with either GP67 or insect hemolin signal peptide for plant protein secretion expression in insect cells. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments