Transcripts Mapping by 3 prime Rapid Amplification of CDNA Ends | Protocol Preview скачать в хорошем качестве

Transcripts Mapping by 3 prime Rapid Amplification of CDNA Ends | Protocol Preview

Watch the Full Video at https://www.jove.com/v/57318/adapting.... Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer - a 2 minute Preview of the Experimental Protocol Chioniso Patience Masamha, Zachery Todd Butler University, Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences; The two different 3' rapid amplification of cDNA ends (3' RACE) protocols described here make use of two different DNA polymerases to map sequences that include a segment of the open reading frame (ORF), the stop codon, and the entire 3' UTR of a transcript using RNA obtained from different cancer cell lines. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments