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Watch the Full Video at https://www.jove.com/v/60542/developm.... Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss - a 2 minute Preview of the Experimental Protocol Annekatrin Holzhey, Svenja Sonntag, Johannes Rendenbach, Justus S. Ernesti, Vinodh Kakkassery, Salvatore Grisanti, Fred Reinholz, Sebastian Freidank, Alfred Vogel, Mahdy Ranjbar University of Lübeck, Laboratory for Angiogenesis and Ocular Cell Transplantation; University of Lübeck, Department of Ophthalmology; University of Lübeck, Institute of Biomedical Optics; Here, we present a protocol to detach corneal endothelial cells (CEC) from Descemet’s membrane (DM) using a neodymium:YAG (Nd:YAG) laser as an ex vivo disease model for bullous keratopathy (BK). Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments