Golden Gate Cloning or assembly - Type IIS (BsaI) restriction enzyme based cloning скачать в хорошем качестве

Golden Gate Cloning or assembly - Type IIS (BsaI) restriction enzyme based cloning

References/Resources:   / the_crux   All videos on Genetic Engineering:    • Genetic Engineering (Genes to Genomes)   Golden Gate Cloning or Golden gate assembly is a restriction enzyme based cloning that uses type IIS restriction enzymes. It is a powerful way to clone large numbers of inserts in a single reaction without the need of using multiple enzymes. This video discusses the basis of golden gate assembly or golden gate cloning, and the intuition behind using a type IIS restriction enzyme. I also discuss how you could do golden gate cloning without using Type IIS restriction enzymes. Towards the end, I discuss the syntax involved in working with golden gate assembly or cloning. Jump to your favorite section: 00:00 Type IIS enzymes 01:16 Incompatible overhangs 01:58 How to Golden Gate 05:08 Golden Gate syntax References/Resources:   / the_crux   Suggested video: Restriction Enzyme Cloning:    • Cloning with Restriction Enzymes (5 Levels...   Must watch playlists All videos on Genetic Engineering:    • Genetic Engineering (Genes to Genomes)   All videos on DNA Replication:    • All about DNA Replication!   All videos on Transcription:    • All about Transcription!   All videos on Translation:    • All about Translation!   Consider supporting the growth of this channel by becoming a Patron:   / the_crux   I appreciate your support :)