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Watch the Full Video at https://www.jove.com/v/51341/quantifi.... Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model - a 2 minute Preview of the Experimental Protocol Donna Cvetković, Cameron Glenn-Franklin Goertzen, Moshmi Bhattacharya University of Western Ontario, Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry; University of Western Ontario, Department of Oncology, Schulich School of Medicine and Dentistry; Lawson Health Research Institute,; This article provides detailed methodologies for the use of three-dimensional (3D) assays to quantify breast cancer cell invasion. Specifically, we discuss the procedures required to set up such assays, quantification, and data analysis, as well as methods to examine the loss of membrane integrity that occurs when cells invade. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments