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Watch the Full Video at https://www.jove.com/v/54801/preparat.... Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue - a 2 minute Preview of the Experimental Protocol Jennifer T. Malon, Ling Cao University of New England, Department of Biomedical Sciences, College of Osteopathic Medicine; The development of neuropathic pain involves pathological changes of spinal cord glial cells. A reliable glial culture system derived from adult spinal cord tissue and designed for studying these cells in vitro is lacking. Therefore, we show here how to establish primary mixed glial cultures from adult mouse spinal cord tissue. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments