Understanding RNase H in 60 Seconds скачать в хорошем качестве

Understanding RNase H in 60 Seconds

At SBS Genetech, we provide high-quality RNase H. Explore more at https://www.sbsgenetech.com/store/pro... Ribonuclease H (RNase H) is an endoribonuclease enzyme that specifically degrades the RNA strand of RNA-DNA hybrids. It catalyzes the hydrolysis of the phosphodiester bonds in the RNA component, producing 5' phosphate-terminated oligoribonucleotides and single-stranded DNA. RNase H does not degrade single-stranded nucleic acids, double-stranded DNA, or double-stranded RNA. This enzyme is crucial for various biological processes, including the removal of RNA primers during DNA replication and the degradation of RNA in RNA-DNA hybrids during transcription and reverse transcription. RNase H is widely used in molecular biology applications, such as removing mRNA during second-strand cDNA synthesis, and in oligodeoxyribonucleotide-directed cleavage of RNA. It is typically purified from Escherichia coli and is active under conditions that include 20 mM Tris-HCl (pH 7.5), 100 mM KCl, 10 mM MgCl2, and 0.1 mM DTT. The enzyme's activity is defined by its ability to solubilize 1 nmol of poly(A) in 20 minutes at 37°C.