HUVEC Transfection Optimized with Cytofect™ Kit скачать в хорошем качестве

HUVEC Transfection Optimized with Cytofect™ Kit

High definition live cell imaging (HD-LCI) to visualize real time expression of Green Fluorescent Protein (GFP) in human umbilical vein endothelial cells (HUVEC) after transient transfection using Cytofect™ HUVEC Transfection Kit (Cat. # TF200K, Cell Applications, Inc.). The HUVEC cells (Cat. # 200-05N, Cell Applications, Inc.) light up green due to the intracellular expression of GFP protein. Cytofect™ transfection reagent successfully delivered GFP-expressing vector into the cells, with protein detected as little as 3 hr post-transfection, compared to the 12+ hr often seen with traditional reagents. No significant cell death was observed post-transfection, a crucial indication of very low toxicity for potentially sensitive primary cells. The cells remained motile during the entire course of experiment, further indicating optimal cell health. Time-series imaging was performed in a humidified incubator set to 5% CO2 / 37°C using a Lumascope 620 microscope (Etaluma; www.etaluma.com). LED setup t = 1 sec, F2: lum=6%, g=112%, exposure = 869 units, 1 frame/2 mins. We found the Lumascope 620 offered high resolution and a small footprint that fit well in our standard-size incubator. The unit’s versatility included the ability to image through various cell culture vessels, and power supply via just its USB port. Cell Applications, Inc 6455 Weathers Place San Diego, CA 92121 800-645-0848 (US & Canada) +1 (858) 453-0848 info@cellapplications.com