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Tags: Trichomes, Breeding, Genotype Selection, Cannabinoid Production, Cultivation, Light Cycles, Cannabis Genetics, Yield Samuel Haiden, MSc., presents "Identifying the Ideal "Spectral Diet" for Cannabis Production" at CannMed 23. 0:00 Intro 0:22 What the UCONN Agricultural Biotechnology Lab does 4:09 Achieving Full Potential: Genetic Expression 5:47 Spectral Diet, a Method of Crop Steering 8:00 Shade Avoidance, Phytochrome, and Flowering 10:31 What have previous studies lacked? 12:48 First Experiment 13:39 Results 14:14 Repeat Experiment 14:32 Results 16:01 PHY/CRY Responsive Genes 17:53 Plan for Current Experiment 19:15 Conclusions Cannabis sativa is an annual plant grown for fiber, food, and medicine. Cannabis production is increasing quickly in response to demand for high-THC and CBD flowers for both pharmaceutical and recreational markets. Cannabis growers seek efficient, low-cost methods of increasing the production of these valuable secondary metabolites, and they also seek to develop methods which allow them to replicate a specific “chemotype” or composition of secondary metabolites. Using programmable LED lights and controlled grow tents, we studied the impact of red (R) to far-red (FR) light ratios on high-CBD hemp phenotypes. Our preliminary evidence suggests that by increasing the ratio of red (620-700nm) to far-red (700-750nm) during peak production hours, we may be able increase the accumulation of CBDa and production of floral biomass. Furthermore, constant irradiation of high vs. low R:FR ratios resulted in divergent phenotypes with regards to internodal spacing, leaf surface area, and flower development. This indicates that there is some connection between phytochrome, whose activity is determined by the R:FR ratio of the light being absorbed, and cannabinoid production. When phytochrome is irradiated by red light, it becomes active and localizes to the nucleus, where it affects gene expression. It is possible that specific wavelength ratios (B:G:R:FR) can be used to optimize chemotypes and production- both indoors, by using programmable LED technology, and in greenhouse environments, by utilizing electrochromic greenhouse polymers. More research is needed on optimizing “Spectral diet” for cannabis production, and on the relationship between phytochrome and cannabinoid synthesis. Learn More about CannMed: https://cannmedevents.com/