Cell Signaling Network - Protein Profiling Service скачать в хорошем качестве

Cell Signaling Network - Protein Profiling Service

Systems Biology has emerged as an effective approach to studying complex interactions within biological systems. By studying entire networks or pathways, we can better understand the underlying structure of cell signaling networks and how changes in these networks may affect the flow of biological information. LC Sciences offers a comprehensive Cell Signaling Network Protein Profiling Service. Just send us your cell samples and we’ll perform all the necessary functions from sample assay to data analysis. Our comprehensive service includes: assistance with project designs, synthesis of a custom phosphopeptide binding – peptide microarray, all on-chip binding reactions, detection of the bound proteins using a suitable method, array image scan, and data analysis. The KEGG database is a resource for understanding high-level functions and utilities of the biological system from molecular-level information, especially large-scale molecular datasets generated by high-throughput experimental technologies such as microarrays. KEGG PATHWAY is a collection of manually drawn pathway maps representing our knowledge on the molecular interaction and reaction networks for: metabolism, genetic information processing, cellular and other processes. Once selected, we can detect all pathway proteins containing an SH2 binding domain within your KEGG pathway or pathways of interest. Our PepArray Pro web tool contains panels of phosphotyrosine peptide probes specific for each of the pathway proteins and the PepArray™ microfluidic chip technology enables the total customization of content on each individual microarray to suit your needs. Simply: select the pathways, generate the panel, generate the array, and export the layout file. The layout file can be submitted directly to LC Sciences for synthesis of your custom phosphopeptide microarray. Peptides are synthesized on-chip, not pre-synthesized and spotted. We can synthesize a completely custom one-of-a-kind cell signaling microarray specifically for your experiment , delivering results that cannot be achieved with an off-the-shelf assay. After custom array synthesis, we assay your cellular sample and then perform data analysis with our SVM-PEPARRAY software, the online KEGG & DAVID databases and other tools. With the ability to program new sequence designs on the fly, you can quickly revise microarray design and content to keep experiments moving forward based on previous results. Recently, our phosphopeptide microarray was used to identify phosphoproteins that are pivotal in eliciting cellular responses during the process of normal cells becoming cancerous. These phosphoproteins have the potential as biomarkers to detect early cellular responses as a signature of cancer initiation and progression.