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Isothermal amplification is redefining what’s possible for portable molecular diagnostics — bringing lab-grade sensitivity to the field, the clinic, and even resource-limited settings. In this expert-led masterclass, Dr. Till Adhikary (University of Marburg) and Ejona Gjika (Xpedite Diagnostics) share practical insights and step-by-step guidance on how to design, adapt, and optimize recombinase-based amplification assays (RAA, RT-RAA, RPA) for real-world applications. Through live demonstrations and applied examples, the speakers walk through assay development strategies that shorten time-to-result while maintaining high analytical performance — key to building the next generation of field-ready diagnostic tools. Featured Talks Rapid RT-RAA Workflows for RNA Targets — Dr. Till Adhikary, University of Marburg Designing and optimizing RT-RAA assays for RNA detection — from COVID-19 to emerging infectious diseases. Duplex RAA Assay Design for Pathogen Detection — Ejona Gjika, Xpedite Diagnostics GmbH Building a multiplex-ready RAA workflow for Schistosoma detection and other field-deployable applications. What You’ll Learn • How to design an RT-RAA assay with lateral flow readout • How to adapt an existing PCR assay into RAA format • How to build and fine-tune a duplex RAA assay • How isothermal amplification can power portable, point-of-care diagnostics Connect with us: 🌍 xpedite-dx.com 🔗 LinkedIn: Xpedite Diagnostics 🐦 Twitter/X: @XpediteDx 💬 Have questions or want to share your experience with isothermal amplification? Join the discussion below — your insights help advance the future of field-ready molecular diagnostics.