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Watch the Full Video at https://www.jove.com/v/51291/excitoto.... Excitotoxic Stimulation of Brain Microslices as an In vitro Model of Stroke - a 2 minute Preview of the Experimental Protocol Kathryn A. Skelding, Jacinta M. Arellano, David A. Powis, John A. Rostas The University of Newcastle, School of Biomedical Sciences and Pharmacy, Hunter Medical Research Institute; Southern Cross University, School of Health and Human Sciences; The University of Newcastle, School of Medicine and Public Health; We have developed a brain slice model which can be used to examine molecular mechanisms involved in excitotoxicity-mediated brain injury. This technique generates viable mature brain tissue and reduces animal numbers required for experimentation, whilst keeping the neuronal circuitry, cellular interactions, and postsynaptic compartments partly intact. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments