Whole genome sequencing of 43 cannabis and hemp varietals reveals path to breeding compliant hemp скачать в хорошем качестве

Whole genome sequencing of 43 cannabis and hemp varietals reveals path to breeding compliant hemp

The difference between cannabis and hemp is hotly debated. Many genetic markers exist to differentiate these but few genetic markers predict the plants that will express less than 0.3% THCA. A whole genome sequencing survey of 43 genomes to over 50X coverage on Illumina reveals a CBCAS gene cluster that is frequently deleted in Type I plants but often present in Type III plants. The presence of this extra set of cannabinoid synthase genes is believed to be the cause of “Hot Hemp” and genomics directed breeding could enable and guide the breeding of compliant hemp lines. There is one catch. This multi-megabase CBCAS deletion also contains multiple pathogen response genes (NPF3, NIP1, PIP1, RKF1) suggesting breeding for compliant hemp will enrich for hemp with higher microbial loads and potentially more pathogen exposure. To address this we cloned a Cannabis Pathogen Response gene into Ecoli for fungicidal assays and potential foliar spray development. Tools to detect, and manage endophytes will be discussed.